How many units is 100mcg of a peptide?

It depends on how much bacteriostatic water you used. If you reconstituted 5mg (5000mcg) in 1ml of water, each 0.01ml (1 unit on a U100 syringe) = 50mcg. Use our peptide dosage calculator to find exact units for your dose.

What is the standard BPC-157 dosage?

Common BPC-157 research doses range from 250mcg to 500mcg per injection, typically administered once or twice daily subcutaneously or intramuscularly. Always use the peptide calculator to confirm your exact injection volume in units based on your reconstitution ratio.

What bacteriostatic water should I use for peptide reconstitution?

Use pharmaceutical-grade bacteriostatic water (0.9% benzyl alcohol) for multi-dose vials, as it preserves the peptide for up to 4 weeks refrigerated. Sterile water can be used but only for single-use reconstitution since it has no preservative.

How long do reconstituted peptides last?

Reconstituted peptides stored in a refrigerator (2–8°C) with bacteriostatic water typically remain stable for 4–6 weeks. Lyophilized (dry powder) peptides can last 1–2 years when frozen. Never leave reconstituted peptides at room temperature.

What syringe do I use for peptide injections?

Most peptide researchers use U100 insulin syringes (1ml, 100 units). Each unit on the syringe = 0.01ml. This makes dosing calculations straightforward when using our peptide calculator — your result in units corresponds directly to the syringe markings.

Peptide Calculator 2026 – Reconstitution, Dosage & Injection Volume

Use this free peptide calculator to calculate exact reconstitution ratios, injection volumes in units/ml, and dosages in mcg for BPC-157, TB-500, Semaglutide, CJC-1295, Ipamorelin, and all research peptides. Switch between tabs to reconstitute your vial, calculate your dose, or convert units.

Quick Presets:

Peptide Vial Size (mg)

E.g. 2mg, 5mg, 10mg vial

Bacteriostatic Water to Add (ml)

Most researchers use 1–2ml per vial

Desired Dose per Injection (mcg)

E.g. 250mcg, 500mcg

Syringe Type

💡 Already reconstituted your peptide? Enter your vial details below to find the exact number of units to draw for any dose.

Peptide Amount in Vial (mg)

Bacteriostatic Water Used (ml)

Desired Dose (mcg)

Syringe Type

Injections Per Week

Protocol Duration (weeks)

🔄 Convert between mcg, mg, units, and ml — useful for checking peptide dosage labels and syringe readings.

Conversion Type

Value to Convert

Peptide Calculator: Complete Guide to Reconstitution & Dosage (2026)

Whether you are a researcher working with BPC-157, TB-500, Semaglutide, CJC-1295, or Ipamorelin, accurate peptide reconstitution and dosage calculation is critical. A miscalculated dose can mean administering 2× or 0.5× the intended amount — which affects both safety and research outcomes. This peptide calculator eliminates guesswork by computing exact injection volumes in milliliters and syringe units based on your specific vial size and water volume.

💡 Use the peptide reconstitution calculator above to find your exact injection volume. Enter your vial mg, bacteriostatic water ml, and desired dose mcg — the calculator does the rest instantly.

What Is Peptide Reconstitution?

Lyophilized (freeze-dried) peptides must be dissolved in a sterile solution before use. The process of adding bacteriostatic water (or sterile water) to a dry peptide vial is called reconstitution. The amount of water you add directly determines the concentration of your solution — which is why a peptide reconstitution calculator is essential for accurate dosing.

For example: if you reconstitute a 5mg vial with 2ml of bacteriostatic water, the concentration is 2500 mcg/ml. If you want a 250mcg dose, you need to draw 0.10ml — which equals 10 units on a standard U100 insulin syringe.

How to Use This Peptide Calculator

Enter your vial size in mg (e.g., 5mg, 2mg, 10mg)
Enter how much bacteriostatic water you will add (e.g., 1ml, 2ml)
Enter your desired dose in mcg (e.g., 250mcg, 500mcg)
Select your syringe type (most researchers use U100 insulin syringes)
Click Calculate — you get the exact ml and syringe units to draw

Peptide Reconstitution Formula Explained

Step	Formula	Example (5mg vial + 2ml water)
Convert vial to mcg	mg × 1000 = mcg	5mg × 1000 = 5000mcg
Calculate concentration	Total mcg ÷ ml water = mcg/ml	5000 ÷ 2 = 2500 mcg/ml
Volume for dose (ml)	Desired dose mcg ÷ concentration	250 ÷ 2500 = 0.10ml
Convert to U100 units	ml × 100 = units	0.10 × 100 = 10 units
Doses per vial	Total mcg ÷ dose mcg	5000 ÷ 250 = 20 doses

Common Peptide Reconstitution Reference Chart

Vial Size	BW Added	Concentration	250mcg Dose	500mcg Dose	Doses/Vial
2mg	1ml	2000 mcg/ml	0.125ml (12.5 units)	0.25ml (25 units)	8 / 4
5mg	1ml	5000 mcg/ml	0.05ml (5 units)	0.10ml (10 units)	20 / 10
5mg	2ml	2500 mcg/ml	0.10ml (10 units)	0.20ml (20 units)	20 / 10
10mg	2ml	5000 mcg/ml	0.05ml (5 units)	0.10ml (10 units)	40 / 20
10mg	4ml	2500 mcg/ml	0.10ml (10 units)	0.20ml (20 units)	40 / 20

Peptide Dosage Guide by Compound

BPC-157 Peptide Calculator

BPC-157 (Body Protection Compound) is a 15-amino acid peptide derived from human gastric juice. Typical research protocols use 200–500mcg per injection, once or twice daily, either subcutaneously (SQ) or intramuscularly (IM) near the site of interest. Most vials come in 5mg sizes.

Dose	Vial 5mg + 2ml BW	Draw (U100 Syringe)
200mcg	0.08ml	8 units
250mcg	0.10ml	10 units
500mcg	0.20ml	20 units

TB-500 (Thymosin Beta-4) Peptide Calculator

TB-500 is a synthetic version of Thymosin Beta-4. Research protocols commonly use 2–2.5mg per week during loading phase, then 200–300mcg maintenance doses. TB-500 vials typically come in 5mg.

Dose	Vial 5mg + 2ml BW	Draw (U100 Syringe)
500mcg (loading)	0.20ml	20 units
1mg (loading)	0.40ml	40 units
2.5mg weekly split	0.50ml per dose (5 days)	50 units

CJC-1295 + Ipamorelin Peptide Calculator

CJC-1295 (a GHRH analog) is commonly stacked with Ipamorelin (a ghrelin mimetic) for GH pulse amplification in research. Typical doses are 100–300mcg of each peptide per injection, 1–3× daily.

Peptide	Common Dose	Vial 2mg + 1ml BW	U100 Units
CJC-1295	100mcg	0.05ml	5 units
CJC-1295	200mcg	0.10ml	10 units
Ipamorelin	200mcg	0.10ml	10 units
Ipamorelin	300mcg	0.15ml	15 units

Semaglutide Peptide Calculator

Semaglutide is a GLP-1 receptor agonist used in research for metabolic studies. It is typically available in 3mg or 5mg vials and dosed in mg (not mcg), with research protocols starting at 0.25mg per week and escalating.

Dose	Vial 3mg + 1.5ml BW	Draw (U100 Syringe)
0.25mg (250mcg)	0.125ml	12.5 units
0.5mg (500mcg)	0.25ml	25 units
1mg (1000mcg)	0.50ml	50 units

Subcutaneous (SQ) vs Intramuscular (IM) Injection – Which to Use?

Subcutaneous (SQ)

✅ Injected into fat layer under skin

✅ Common sites: belly, thigh, upper arm

✅ Slower absorption — sustained release

✅ Easier to self-administer

✅ Used for: BPC-157 systemic, Semaglutide, CJC/Ipa

❌ Slower peak plasma concentration

Intramuscular (IM)

✅ Injected directly into muscle tissue

✅ Faster absorption and peak levels

✅ Used for localized healing (BPC-157 near injury)

✅ Common for TB-500

❌ Requires more precise injection technique

❌ More discomfort

Bacteriostatic Water vs Sterile Water for Reconstitution

Feature	Bacteriostatic Water (BW)	Sterile Water
Preservative	0.9% benzyl alcohol	None
Multi-dose use	✅ Yes — up to 28 days	❌ Single use only
Shelf life (reconstituted)	4–6 weeks refrigerated	Use immediately
Best for	Multi-dose peptide vials	Single-dose only
Recommended?	✅ Yes for peptide research	Only if BW unavailable

⚠️ Storage Warning: Always store reconstituted peptides refrigerated (2–8°C). Never freeze reconstituted peptides — this degrades the peptide structure. Lyophilized (dry) peptide vials can be frozen for long-term storage.

How to Read a U100 Insulin Syringe for Peptides

The U100 insulin syringe is the standard tool for peptide injection in research settings. Each unit on the syringe equals 0.01ml. So if your peptide calculator tells you to draw 10 units, that means drawing to the 0.10ml mark.

1 unit = 0.01ml
5 units = 0.05ml
10 units = 0.10ml
20 units = 0.20ml
50 units = 0.50ml
100 units = 1.0ml (full syringe)

For small doses (under 5 units), consider diluting your vial further — adding more bacteriostatic water so you are drawing a larger, easier-to-measure volume. Our peptide reconstitution calculator shows you how this affects your concentration automatically.

Frequently Asked Questions – Peptide Calculator

Q: How do I reconstitute a peptide with bacteriostatic water?

A: Using a clean alcohol swab, clean the rubber stopper on both the peptide vial and the BW vial. Draw the desired amount of bacteriostatic water into your syringe. Insert the needle into the peptide vial and slowly push the water down the glass wall — never directly onto the powder. Let the peptide dissolve on its own without shaking. Swirl gently if needed. Store in refrigerator immediately after.

Q: What happens if I add too much or too little water?

A: Adding too much water lowers the concentration — meaning you need to draw a larger volume per dose, which is sometimes preferable for accuracy. Adding too little water raises concentration — meaning very small, hard-to-measure injection volumes. Use our peptide calculator to find the optimal water volume for your desired dose-to-unit ratio.

Q: Is there a peptide calculator app?

A: Our web-based peptide reconstitution calculator works on all devices including mobile — no app download needed. Simply bookmark this page for quick access before every injection session.

Q: How many mcg are in 1mg of peptide?

A: There are exactly 1000 mcg in 1mg. So a 5mg vial contains 5000 mcg total. A 2mg vial contains 2000 mcg. Use this to quickly estimate how many doses are in your vial based on your dose per injection.

Q: Can I mix two peptides in the same syringe?

A: CJC-1295 and Ipamorelin are commonly combined in research. As long as both are reconstituted separately, they can be drawn into the same syringe for a single injection. Always calculate each dose separately using the peptide calculator to ensure accurate dosing of both compounds.

Q: How many vials of BPC-157 do I need for a 12-week protocol?

A: At 250mcg twice daily (500mcg/day) for 12 weeks (84 days): total = 42,000mcg = 42mg of BPC-157. At 5mg per vial, you need approximately 8–9 vials. Use the dosage tab in our peptide calculator to plan your total supply needs.

Last Updated: 2026. This peptide calculator is provided for educational and research planning purposes only. All peptide compounds referenced are research chemicals. Consult a licensed medical professional before any use. Peptides are not approved by the FDA for human use outside of specific clinical contexts.